[HTML][HTML] Benchmarking in vitro tissue-engineered blood–brain barrier models

JG DeStefano, JJ Jamieson, RM Linville… - Fluids and Barriers of …, 2018 - Springer
The blood–brain barrier (BBB) plays a key role in regulating transport into and out of the
brain. With increasing interest in the role of the BBB in health and disease, there have been …

Cerebrovascular plasticity: Processes that lead to changes in the architecture of brain microvessels

MI Bogorad, JG DeStefano… - Journal of Cerebral …, 2019 - journals.sagepub.com
The metabolic demands of the brain are met by oxygen and glucose, supplied by a complex
hierarchical network of microvessels (arterioles, capillaries, and venules). Transient …

Human iPSC-derived blood-brain barrier microvessels: validation of barrier function and endothelial cell behavior

RM Linville, JG DeStefano, MB Sklar, Z Xu, AM Farrell… - Biomaterials, 2019 - Elsevier
Microvessels of the blood-brain barrier (BBB) regulate transport into the brain. The highly
specialized brain microvascular endothelial cells, a major component of the BBB, express tight …

[HTML][HTML] Effect of shear stress on iPSC-derived human brain microvascular endothelial cells (dhBMECs)

JG DeStefano, ZS Xu, AJ Williams, N Yimam… - Fluids and Barriers of …, 2017 - Springer
Background The endothelial cells that form the lumen of capillaries and microvessels are an
important component of the blood–brain barrier. Cell phenotype is regulated by transducing …

Modeling hyperosmotic blood–brain barrier opening within human tissue-engineered in vitro brain microvessels

RM Linville, JG DeStefano, MB Sklar… - Journal of Cerebral …, 2020 - journals.sagepub.com
As the majority of therapeutic agents do not cross the blood–brain barrier (BBB), transient
BBB opening (BBBO) is one strategy to enable delivery into the brain for effective treatment of …

Real-time quantification of endothelial response to shear stress and vascular modulators

JG DeStefano, A Williams, A Wnorowski… - Integrative …, 2017 - academic.oup.com
Quiescence is commonly used to describe the inactive state of endothelial cells (ECs) in
monolayers that have reached homeostasis. Experimentally quiescence is usually described in …

Reversible blood-brain barrier opening utilizing the membrane active peptide melittin in vitro and in vivo

…, A Komin, X Lan, JG DeStefano, C Chu, G Liu… - Biomaterials, 2021 - Elsevier
The blood-brain barrier (BBB) tightly controls entry of molecules and cells into the brain,
restricting the delivery of therapeutics. Blood-brain barrier opening (BBBO) utilizes reversible …

[HTML][HTML] Three-dimensional microenvironment regulates gene expression, function, and tight junction dynamics of iPSC-derived blood–brain barrier microvessels

…, RF Nerenberg, J Zhou, R Ye, JG DeStefano… - Fluids and Barriers of …, 2022 - Springer
The blood–brain barrier (BBB) plays a pivotal role in brain health and disease. In the BBB,
brain microvascular endothelial cells (BMECs) are connected by tight junctions which …

Long-term cryopreservation preserves blood–brain barrier phenotype of iPSC-derived brain microvascular endothelial cells and three-dimensional microvessels

RM Linville, JG DeStefano, RF Nerenberg… - Molecular …, 2020 - ACS Publications
Brain microvascular endothelial cells derived from induced pluripotent stem cells (dhBMECs)
are a scalable and reproducible resource for studies of the human blood–brain barrier, …

The Vaccine Safety Datalink: successes and challenges monitoring vaccine safety

…, LA Jackson, SB Omer, SJ Jacobsen, F DeStefano - Vaccine, 2014 - Elsevier
… mellitus No association with any of recommended childhood vaccines DeStefano et al. [39] …
B vaccines may be due to healthcare utilization bias or information bias DeStefano et al. [40] …