Cyclic dinucleotides are an important class of signaling molecules that regulate a wide variety
of pathogenic responses in bacteria, but tools for monitoring their regulation in vivo are …

The vitamin folate is required for methionine homeostasis in all organisms. In addition to its
role in protein synthesis, methionine is the precursor to S-adenosyl-methionine (SAM), which …

We determined that over 40 spliceosomal proteins are conserved between many fungal
species and humans but were lost during the evolution of S. cerevisiae, an intron-poor yeast …

Tools to understand how the spliceosome functions in vivo have lagged behind advances in
the structural biology of the spliceosome. Here, methods are described to globally profile …

Eukaryotic protein synthesis generally initiates at a start codon defined by an AUG and its
surrounding Kozak sequence context, but the quantitative importance of this context in different …

Homology Directed Repair (HDR) enables precise genome editing, but the implementation
of HDR-based therapies is hindered by limited efficiency in comparison to methods that …

Cyclic di-GMP (c-di-GMP) is a second messenger that is important in regulating bacterial
physiology and behavior, including motility and virulence. Many questions remain about the …

The human pathogenic yeast Cryptococcus neoformans silences transposable elements
using endo-siRNAs and an Argonaute, Ago1. Endo-siRNAs production requires the RNA-…

Eukaryotic protein synthesis initiates at a start codon defined by an AUG and its surrounding
Kozak sequence context, but studies of S. cerevisiae suggest this context is of little …

PN and HDM designed the study. JSL performed the experiments in Fig. S6. CH and JGD
assisted in data analysis for RNAseq and Ribosome profiling respectively. XZ and CF …