Role of multicellular aggregates in biofilm formation

KN Kragh, JB Hutchison, G Melaugh, C Rodesney… - MBio, 2016 - Am Soc Microbiol
In traditional models of in vitro biofilm development, individual bacterial cells seed a surface,
multiply, and mature into multicellular, three-dimensional structures. Much research has …

[HTML][HTML] Shaping the growth behaviour of biofilms initiated from bacterial aggregates

G Melaugh, J Hutchison, KN Kragh, Y Irie, A Roberts… - PloS one, 2016 - journals.plos.org
Bacterial biofilms are usually assumed to originate from individual cells deposited on a
surface. However, many biofilm-forming bacteria tend to aggregate in the planktonic phase so …

The Pseudomonas aeruginosa PSL Polysaccharide Is a Social but Noncheatable Trait in Biofilms

Y Irie, AEL Roberts, KN Kragh, VD Gordon, J Hutchison… - MBio, 2017 - Am Soc Microbiol
Extracellular polysaccharides are compounds secreted by microorganisms into the surrounding
environment, and they are important for surface attachment and maintaining structural …

Zwitterionic surface chemistry enhances detachment of bacteria under shear

MK Shave, Y Zhou, J Kim, YC Kim, J Hutchison… - Soft matter, 2022 - pubs.rsc.org
The ubiquitous nature of microorganisms, especially of biofilm-forming bacteria, makes
biofouling a prevalent challenge in many settings, including medical and industrial environments …

Single-cell control of initial spatial structure in biofilm development using laser trapping

JB Hutchison, CA Rodesney, KS Kaushik, HH Le… - Langmuir, 2014 - ACS Publications
Biofilms are sessile communities of microbes that are spatially structured by an embedding
matrix. Biofilm infections are notoriously intractable. This arises, in part, from changes in the …

Change of line tension in phase-separated vesicles upon protein binding

JB Hutchison, RM Weis, AD Dinsmore - Langmuir, 2012 - ACS Publications
We measured the effect of a model membrane-binding protein on line tension and
morphology of phase-separated lipid-bilayer vesicles. We studied giant unilamellar vesicles …

Osmotically-induced tension and the binding of N-BAR protein to lipid vesicles

JB Hutchison, APKKK Mudiyanselage, RM Weis… - Soft Matter, 2016 - pubs.rsc.org
The binding affinity of a curvature-sensing protein domain (N-BAR) is measured as a function
of applied osmotic stress while the membrane curvature is nearly constant. Varying the …

Increased production of the extracellular polysaccharide Psl can give a growth advantage to Pseudomonas aeruginosa in low-iron conditions

J Hutchison, K Kaushik, CA Rodesney, T Lilieholm… - BioRxiv, 2018 - biorxiv.org
In infections, biofilm formation is associated with a number of fitness advantages, such as
resistance to antibiotics and to clearance by the immune system. Biofilm formation has also …

[PDF][PDF] Shaping the growth behaviour of bacterial aggregates in biofilms

G Melaugh, J Hutchison, KN Kragh, Y Irie… - arXiv preprint arXiv …, 2015 - researchgate.net
Bacterial biofilms are usually assumed to originate from individual cells deposited on a
surface. However, many biofilm-forming bacteria tend to aggregate in the planktonic phase …

Bacteriophage Φ6 (Phi6) as a Surrogate for Enveloped Viral pathogens in Standard and Long-lasting Virucidal Efficacy Tests

JB Hutchison, C Plummer, G Garner, A Sehgal… - 2021 - researchsquare.com
Abstract Development of novel antimicrobials capable of providing long-lasting disinfection
on surfaces requires the implementation of new standardized methods to support claims …