As developmental biologists in the age of genome editing, we now have access to an ever-increasing
array of tools to manipulate endogenous gene expression. The auxin-inducible …

Type III secretion systems (T3SS) are essential for virulence in dozens of pathogens, but are
not required for growth outside the host. Therefore, the T3SS of many bacterial species are …

As developmental biologists in the age of genome editing, we now have access to an ever-increasing
array of tools to manipulate endogenous gene expression. By combining CRISPR/…

As developmental biologists in the age of genome editing, we now have access to an
everincreasing array of tools to manipulate endogenous gene expression. The auxin-inducible …

The auxin-inducible degron (AID) system is a widely-used system for conditional protein
depletion. During the course of an experiment, we depleted the nuclear hormone receptor …

In sexually reproducing metazoans, spermatogenesis is the process by which uncommitted
germ cells give rise to haploid sperm. Work in model systems has revealed mechanisms …

The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to augment …

Spermatogenesis is the process through which mature male gametes are formed and is
necessary for the transmission of genetic information. While much work has established how …

Adjacent alternative 3′ splice sites, those separated by ≤18 nucleotides, provide a unique
problem in the study of alternative splicing regulation; there is overlap of the cis-elements …

Lysosomes are an important organelle required for the degradation of a range of cellular
components. Lysosome function is critical for development and homeostasis as dysfunction can …