Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences
are often underestimated. Damage to cellular macromolecules upon excitation light …

Cell adhesion to the extracellular matrix is fundamental to metazoan multicellularity and is
accomplished primarily through the integrin family of cell-surface receptors. Integrins are …

There is increasing evidence to suggest that splicing decisions are largely made when the
nascent RNA is still associated with chromatin. Here we demonstrate that activity of histone …

The conditional and reversible depletion of proteins by auxin-mediated degradation is a
powerful tool to investigate protein functions in cells and whole organisms. However, its wider …

The concomitant occurrence of tissue growth and organization is a hallmark of organismal
development 1 , 2 – 3 . This often means that proliferating and differentiating cells are found at …

The arrangement of neurons into distinct layers is critical for neuronal connectivity and function.
During development, most neurons move from their birthplace to the appropriate layer, …

Apical radial glia (aRG), the stem cells in developing neocortex, are unique bipolar epithelial
cells, extending an apical process to the ventricle and a basal process to the basal lamina. …

Light sheet fluorescence microscopy (LSFM) is gaining more and more popularity as a method
to image embryonic development. The main advantages of LSFM compared to confocal …

β1-integrins mediate cell–matrix interactions and their trafficking is important in the dynamic
regulation of cell adhesion, migration and malignant processes, including cancer cell …

Since the late 1960s, FCM has given the biologist the ability to interrogate both normal and
diseased biological conditions at the single-cell level in a statistically relevant way (1). For …