Native mass spectrometry (MS) is increasingly used to provide complementary data to electron
microscopy (EM) for protein structure characterization. Beyond the ability to provide mass …

ADP-ribosylation (ADPr) is a post-translational modification that plays pivotal roles in a wide
range of cellular processes. Mass spectrometry (MS)-based analysis of ADPr under …

Monoclonal antibodies (mAbs) are important therapeutic glycoproteins, but their large size
and structural complexity make them difficult to rapidly characterize. Top-down mass …

Photoactivation and photodissociation have long proven to be useful tools in tandem mass
spectrometry, but implementation often involves cumbersome and potentially dangerous …

Tethering is a screening technique for discovering small-molecule fragments that bind to pre-determined
sites via formation of a disulphide bond. Tethering screens traditionally rely …

Allosteric binding events play a critical role in the formation and stability of transcriptional
activator–coactivator complexes, perhaps in part due to the often intrinsically disordered nature …

Capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) has been
recognized as an efficient approach for top-down proteomics recently for its high-capacity …

The kinase-inducible domain (KIX) of the transcriptional coactivator CBP binds multiple
transcriptional regulators through two allosterically connected sites. Establishing a method for …

The GACKIX activator binding domain has been a compelling target for small‐molecule probe
discovery because of the central role of activator–GACKIX complexes in diseases ranging …

This revision of the classification of unicellular eukaryotes updates that of Levine et al. (1980)
for the protozoa and expands it to include other protists. Whereas the previous revision …