We describe automated technologies to probe the structure of neural tissue at nanometer
resolution and use them to generate a saturated reconstruction of a sub-volume of mouse …

We generated transgenic mice in which red, green, yellow, or cyan fluorescent proteins (together
termed XFPs) were selectively expressed in neurons. All four XFPs labeled neurons in …

Although fluorescence microscopy permeates all of cell and molecular biology, most biologists
have little experience with the underlying photophysical phenomena. Understanding the …

A central aim of neuroscience is to map neural circuits, in order to learn how they account
for mental activities and behaviours and how alterations in them lead to neurological and …

Detailed analysis of neuronal network architecture requires the development of new methods.
Here we present strategies to visualize synaptic circuits by genetically labelling neurons …

The relation between the structure of the nervous system and its function is more poorly
understood than the relation between structure and function in any other organ system. We …

… JW Lichtman is a postdoctoral fellow in the Department of Neurobiology at Harvard
Medical School, Boston, Massachusetts 02115. … Lichtman … Purves and JW Lichtman …

▪ Abstract We describe the formation, maturation, elimination, maintenance, and regeneration
of vertebrate neuromuscular junctions (NMJs), the best studied of all synapses. The NMJ …

The postsynaptic apparatus of the skeletal neuromuscular junction, like that of other synapses,
contains a high-density patch of neurotransmitter receptors that is closely associated with …

Biological specimens are intrinsically three dimensional; however, because of the obscuring
effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to …