Improvements in purification of membrane-associated methane monooxygenase (pMMO)
have resulted in preparations of pMMO with activities more representative of physiological …

Cloning by homologous recombination (HR) in Saccharomyces cerevisiae is an extremely
efficient and cost-effective alternative to other methods of recombinant DNA technologies. …

10.7554/eLife.23845.001 Biofilms are communities of microorganisms attached to a surface
or each other. Biofilm-associated cells are the etiologic agents of recurrent Staphylococcus …

Staphylococcus aureus is a prominent bacterial pathogen that is known to agglutinate in the
presence of human plasma to form stable clumps. There is increasing evidence that …

Building iron-sulfur (Fe-S) clusters and assembling Fe-S proteins are essential actions for
life on Earth. The three processes that sustain life, photosynthesis, nitrogen fixation, and …

Staphylococcus aureus is a versatile bacterial pathogen that can cause significant disease
burden and mortality. Like other pathogens, S. aureus must adapt to its environment to …

Staphylococcus aureus exhibits an unusually high level of osmotolerance and Na + tolerance,
properties that support survival in various host niches and in preserved foods. The genetic …

The acquisition and metabolism of iron ( Fe ) by the human pathogen S taphylococcus aureus
is critical for disease progression. S . aureus requires Fe to synthesize inorganic cofactors …

Staphylococcus aureus is an important pathogen that leads to significant disease through
multiple routes of infection. We recently published a transposon sequencing (Tn-seq) screen …

… of the Sulfolobales and the Desulfurococcales (ES Boyd, unpublished data). Thus, the ancestral
… the scope of this review (ES Boyd, JM Boyd, and FW Outten, manuscript in preparation). …