Defects in the assembly of dolichol‐linked oligosaccharide or its transfer to proteins result in
severe, multi‐system human diseases called Type I congenital disorders of glycosylation. …

Dolichol monophosphate (Dol‐P) functions as an obligate glycosyl carrier lipid in protein
glycosylation reactions. Dol‐P is synthesized by the successive condensation of isopentenyl …

Plants respond to infection by accumulating low-molecular-weight antimicrobial stress
metabolites called phytoalexins. The phytoalexins are generally lipophilic substances that are …

The assembly of many bacterial cell surface polysaccharides requires the transbilayer
movement of polyisoprenoid-linked saccharide intermediates across the cytoplasmic membrane. …

Cell wall glycopolymers on the surface of Gram-positive bacteria are fundamental to bacterial
physiology and infection biology. Here we identify gacH, a gene in the Streptococcus …

The Lec35 gene product (Lec35p) is required for utilization of the mannose donor mannose-P-dolichol
(MPD) in synthesis of both lipid-linked oligosaccharides (LLOs) and …

Congenital disorders of glycosylation (CDG) arise from pathogenic mutations in over 100
genes leading to impaired protein or lipid glycosylation. ALG1 encodes a β1,4 …

Escherichia coli strain O157 produces an O-antigen with the repeating tetrasaccharide unit α-d-PerNAc-α-l-Fuc-β-d-Glc-α-d-GalNAc,
preassembled on undecaprenyl pyrophosphate (…

The ability of Nicotiana tabacum cell cultures to utilize farnesol (F-OH) for sterol and
sesquiterpene biosynthesis was investigated. [ 3 H]F-OH was readily incorporated into sterols by …

… We now show that a specific ER protein(s), but not Rft1, is required to flip Man 5 GlcNAc 2
-PP-Dol in reconstituted vesicles. Rft1 may have a critical accessory role in translocating Man …