To equalize X-chromosome dosages between the sexes, the female mammal inactivates one
of her two X chromosomes. X-chromosome inactivation (XCI) is initiated by expression of …

The presence of two active X chromosomes (XaXa) is a hallmark of the ground state of
pluripotency specific to murine embryonic stem cells (ESCs). Human ESCs (hESCs) invariably …

Single-cell sequencing methods have emerged as powerful tools for identification of
heterogeneous cell types within defined brain regions. Application of single-cell techniques to study …

A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated
from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is …

Mobile elements are DNA sequences that can change their position (retrotranspose) within
the genome. Although its biological function is largely unappreciated, DNA derived from …

BACKGROUND Elucidating the genetic architecture of neuropsychiatric disorders remains
a major scientific and medical challenge. Emerging genomic technologies now permit the …

The healthy human brain is a mosaic of varied genomes. Long interspersed element-1 (LINE-1
or L1) retrotransposition is known to create mosaicism by inserting L1 sequences into …

CTCF is a master regulator that plays important roles in genome architecture and gene
expression. How CTCF is recruited in a locus-specific manner is not fully understood. Evidence …

Most studies of gene expression in the brains of individuals with schizophrenia have focused
on cortical regions, but subcortical nuclei such as the striatum are prominently implicated in …

Despite widespread interest in using human induced pluripotent stem cells (hiPSCs) in
neurological disease modeling, a suitable model system to study human neuronal connectivity is …