We introduce a method for optically imaging intracellular proteins at nanometer spatial
resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were …

Since the advent of the green fluorescent protein, the subcellular localization, mobility,
transport routes and binding interactions of proteins can be studied in living cells. Live cell …

The ability to visualize, track, and quantify molecules and events in living cells with high spatial
and temporal resolution is essential for understanding biological systems. Only recently …

The organization of the eukaryotic cell into discrete membrane-bound organelles allows for
the separation of incompatible biochemical processes, but the activities of these organelles …

THE definition of cellular organelles has evolved over the last hundred years largely driven
by morphologic observations, but more recently has been supplemented and complemented …

Starvation-induced autophagosomes engulf cytosol and/or organelles and deliver them to
lysosomes for degradation, thereby resupplying depleted nutrients. Despite advances in …

Understanding molecular-scale architecture of cells requires determination of 3D locations
of specific proteins with accuracy matching their nanometer-length scale. Existing electron …

We combined photoactivated localization microscopy (PALM) with live-cell single-particle
tracking to create a new method termed sptPALM. We created spatially resolved maps of single…

Introduction In vivo imaging provides a window into the spatially complex, rapidly evolving
physiology of the cell that structural imaging alone cannot. However, observing this …

… no effect on the assembly of TCR subunits into multimeric complexes (Lippincott-Schwartz, …
a chains are subjected to ER degradation, which BFA does not inhibit (Lippincott-Schwartz …