Although much biological research depends upon species diagnoses, taxonomic expertise
is collapsing. We are convinced that the sole prospect for a sustainable identification …

With millions of species and their life-stage transformations, the animal kingdom provides a
challenging target for taxonomy. Recent work has suggested that a DNA-based identification …

Although commercial kits are available for automated DNA extraction, ‘artisanal’ protocols
are not. In this study, we present a silica‐based method that is sensitive, inexpensive and …

Large-scale DNA barcoding projects are now moving toward activation while the creation of
a comprehensive barcode library for eukaryotes will ultimately require the acquisition of …

DNA barcoding systems employ a short, standardized gene region to identify species. A 648-bp
segment of mitochondrial cytochrome c oxidase 1 (CO1) is the core barcode region for …

The Barcode of Life initiative represents an ambitious effort to develop an identification system
for eukaryotic life based on the analysis of sequence diversity in short, standardized gene …

Recent estimates suggest that the global insect fauna includes fewer than six million species,
but this projection is very uncertain because taxonomic work has been limited on some …

This study reports DNA barcodes for more than 1300 Lepidoptera species from the eastern
half of North America, establishing that 99.3 per cent of these species possess diagnostic …

DNA barcoding protocols require the linkage of each sequence record to a voucher specimen
that has, whenever possible, been authoritatively identified. Natural history collections …

Background Although high-throughput sequencers (HTS) have largely displaced their Sanger
counterparts, the short read lengths and high error rates of most platforms constrain their …