The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. It is currently
believed that four to six crypt stem cells reside at the +4 position immediately above the …

Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential
aspects of organ structure and function. Here, we describe a method to establish long‐term…

In many organisms the allocation of primordial germ cells (PGCs) is determined by the
inheritance of maternal factors deposited in the egg. However, in mammals, inductive cell …

The study of gastric epithelial homeostasis and cancer has been hampered by the lack of
stem cell markers and in vitro culture methods. The Wnt target gene Lgr5 marks stem cells in …

Breast cancer (BC) comprises multiple distinct subtypes that differ genetically, pathologically,
and clinically. Here, we describe a robust protocol for long-term culturing of human …

Crypt stem cells represent the cells of origin for intestinal neoplasia. Both mouse and human
intestinal stem cells can be cultured in medium containing the stem-cell-niche factors WNT, …

Lgr5 + intestinal stem cells generate enterocytes and secretory cells. Secretory lineage
commitment requires Notch silencing. The Notch ligand Dll1 is expressed by a subset of …

Ovarian cancer (OC) is a heterogeneous disease usually diagnosed at a late stage. Experimental
in vitro models that faithfully capture the hallmarks and tumor heterogeneity of OC are …

Intestinal crypts display robust regeneration upon injury. The relatively rare secretory precursors
can replace lost stem cells, but it is unknown if the abundant enterocyte progenitors that …

The mammalian liver possesses a remarkable regenerative ability. Two modes of damage
response have been described: (1) The "oval cell" response emanates from the biliary tree …