Background: Polyene macrolides are a class of large macrocyclic polyketides that interact
with membrane sterols, having antibiotic activity against fungi but not bacteria. Their rings …

The three giant multifunctional polypeptides of the rapamycin (Rp)-producing polyketide
synthase (RAPS1, RAPS2 and RAPS3) have recently been shown to contain 14 separate sets, …

Pimaricin (natamycin) is a small polyene macrolide antibiotic used worldwide. This efficient
antimycotic and antiprotozoal agent, produced by several soil bacterial species of the genus …

Modular polyketide synthases are multienzymes responsible for the biosynthesis of a large
number of clinically important natural products. They contain multiple sets, or modules, of …

The amino acid sequences of a large number of polyketide synthase domains that catalyse
the transacylation of either methylmalonyl‐CoA or malonyl‐CoA onto acyl carrier protein (…

Sequencing of the DNA region on the left fringe of the pimaricin gene cluster revealed the
presence of a 3.6-kb gene, pimR, whose deduced product (1,198 amino acid residues) was …

The biosynthesis of the antifungal pimaricin in Streptomyces natalensis is very sensitive to
phosphate regulation. Concentrations of inorganic phosphate above 1mM drastically reduced …

The macrocyclic polyketides rapamycin and FK506 are potent immunosuppressants that
prevent T-cell proliferation through specific binding to intracellular protein receptors (…

Analysis of the gene cluster from Streptomyces hygroscopicus that governs the biosynthesis
of the polyketide immuno-suppressant rapamycin (Rp) has revealed that it contains three …

The biosynthetic gene cluster for the 26-membered ring of the polyene macrolide pimaricin
extends for about 110 kilobase pairs of contiguous DNA in the genome of Streptomyces …