Programmable control over an addressable global regulator would enable simultaneous
repression of multiple genes and would have tremendous impact on the field of synthetic …

Engineering protein translation machinery to incorporate noncanonical amino acids (ncAAs)
into proteins has advanced applications ranging from proteomics to single-molecule studies…

Archaeal pyrrolysyl-tRNA synthetases (PylRSs) have been used to genetically encode over
200 distinct noncanonical amino acids (ncAAs) in proteins in Escherichia coli and …

Protein expression with genetically encoded noncanonical amino acids (ncAAs) benefits a
broad range of applications, from the discovery of biological therapeutics to fundamental …

Numerous applications of noncanonical amino acids (ncAAs) in basic biology and therapeutic
development require efficient protein biosynthesis using an expanded genetic code. …

Genetic code expansion is a powerful approach for advancing critical fields such as biological
therapeutic discovery. However, the machinery for genetically encoding noncanonical …

Genetic code expansion has allowed for extraordinary advances in enhancing protein
chemical diversity and functionality, but there remains a critical need for understanding and …

Yeast display has been used to advance many critical research areas, including the discovery
of unique protein binders and biological therapeutics. In parallel, noncanonical amino …

… submitted by Jessica T. Stieglitz in partial fulfillment of the requirements for the degree of
Doctor of Philosophy in Chemical Engineering Tufts University August 2021 © 2021, Jessica T. …

… Stieglitz† and James A. Van Deventer*, †, ‡ … [32] Potts, KA, Stieglitz, JT, Lei, M., Van
Deventer, JA (2020) Reporter system architecture … [38] Potts, KA, Stieglitz, JT, Lei, M., and …