Optogenetic tools for controlling gene expression are ideal for tuning synthetic biological
networks due to the exquisite spatiotemporal control available with light. Here we develop an …

Introduction Controlling gene expression is a fundamental goal of basic and synthetic biology
because it allows insight into cellular function and control of cellular activity. We explored …

… , and always encouraging me to get out of my comfort zone. Additionally, I thank Luke Voce
… Lastly, in Chapter 7, I discuss my conclusions from this work and future directions for these …

Synthetic biology in Saccharomyces cerevisiae or budding yeast has proved indispensable
in metabolic engineering, gene circuit engineering, and molecular and cell biology. These …

Molecules and combinations of molecules are the natural communication currency of
microbes; microbes have evolved and been engineered to sense a variety of compounds, often …

Eukaryotic transcription factors (TFs) perform complex and combinatorial functions within
transcriptional networks. Here, we present a synthetic framework for systematically constructing …