Efficient and safe methods for delivering exogenous genetic material into tissues must be
developed before the clinical potential of gene therapy will be realized. Recently, in vivo …

Introduction Reports of high rates of primary microcephaly and Guillain–Barré syndrome
associated with Zika virus infection in French Polynesia and Brazil have raised concerns that …

SARS-CoV-2 infection is required for COVID-19, but many signs and symptoms of COVID-19
differ from common acute viral diseases. SARS-CoV-2 infection is necessary but not …

Naked DNA injection with electropermeabilization (EP) is a promising method for nucleic acid
vaccination (NAV) and in vivo gene therapy. Skin is an ideal target for NAV due to ease of …

The ongoing Zika virus epidemic in the Americas and the observed association with both
fetal abnormalities (primary microcephaly) and adult autoimmune pathology (Guillain–Barré …

Preclinical in vivo rodent, porcine, and primate experiments aimed at enhancing nonviral
transgene delivery to skin have been performed. These investigations have identified a …

Background Nuclease activity present within respiratory tissues contributes to the rapid
clearance of injected DNA and therefore may reduce the transfection activity of directly injected …

Simple, nontoxic, and pharmaceutically defined methods for genetic modification of
respiratory tissues may enable development of a variety of molecular medicines. Clinical …

The purpose of this study was to assess the perceptions of academic scientists on the
granting and peer review processes at the National Institutes of Health (NIH) and to determine …

Gene therapy may be defined as providing clinical benefit via the administration of recombinant
viruses or nucleic acid formulations that encode transgenic proteins. Implementation of …