Building expression constructs for transgenesis is one of the fundamental day-to-day tasks
in modern biology. Traditionally it is based on a multitude of type II restriction endonucleases …

Stem cells in plants constantly supply daughter cells to form new organs and are expected
to safeguard the integrity of the cells from biological invasion. Here, we show how stem cells …

The development of technologies for the genetic manipulation of mitochondrial genomes
remains a major challenge. Here we report a method for the targeted introduction of mutations …

The development of technologies for the stable genetic transformation of plastid (chloroplast)
genomes has been a boon to both basic and applied research. However, extension of the …

Genome editing continues to revolutionize biological research. Due to its simplicity and
flexibility, CRISPR/Cas-based editing has become the preferred technology in most systems. …

Despite the independent evolution of multicellularity in plants and animals, the basic
organization of their stem cell niches is remarkably similar. Here, we report the genome-wide …

10.7554/eLife.17023.001 A major feature of embryogenesis is the specification of stem cell
systems, but in contrast to the situation in most animals, plant stem cells remain quiescent …

Fusion proteins derived from transcription activator-like effectors (TALEs) have emerged as
genome editing tools for mitochondria. TALE nucleases (TALENs) have been applied to …

With CR–RT–PCR as primary approach we mapped the 5′ and 3′ transcript ends of all
mitochondrial protein-coding genes in Arabidopsis thaliana . Almost all transcripts analyzed …

To maintain the balance between long-term stem cell self-renewal and differentiation,
dynamic signals need to be translated into spatially precise and temporally stable gene …