The SPIDER system has evolved into a comprehensive tool set for image processing, making
use of modern graphics interfacing in the VMS and UNIX environment. SPIDER and WEB …

We have developed a new technique of analysis that allows automatic classification of molecule
images according to subtle differences. Computer alignment and multivariate statistical …

Electron tomography has become a standard technique with applications in cell biology,
structural biology, and materials science. This definitive work provides a comprehensive …

▪ Abstract Cryo-electron microscopy (cryo-EM) of biological molecules in single-particle (ie,
unordered, nonaggregated) form is a new approach to the study of molecular assemblies, …

Cryoelectron microscopy of biological molecules is among the hottest growth areas in
biophysics and structural biology at present, and Frank is arguably the most distinguished …

During the ribosomal translocation, the binding of elongation factor G (EF-G) to the
pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S …

THE ribosome is formed by assembly of proteins and nucleic acids, and synthesizes proteins
according to genetic instructions in all organisms. Many of the biochemical steps of this …

In vitro assembled yeast ribosome-nascent chain complexes (RNCs) containing a signal
sequence in the nascent chain were immunopurified and reconstituted with the purified protein-…

The ribosome is a macromolecular assembly that is responsible for protein biosynthesis
following genetic instructions in all organisms. It is composed of two unequal subunits: the …

Over 73,000 projections of the E. coli ribosome bound with formyl-methionyl initiator tRNA f
Met were used to obtain an 11.5 Å cryo-electron microscopy map of the complex. This map …