Kinesin-1 is an ATP-driven, processive motor that transports cargo along microtubules in a
tightly regulated stepping cycle. Efficient gating mechanisms ensure that the sequence of …

10.7554/eLife.07403.001 Kinesin-1 is a dimeric motor that transports cargo along microtubules,
taking 8.2-nm steps in a hand-over-hand fashion. The ATP hydrolysis cycles of its two …

The bacterial adaptive immune system CRISPR–Cas9 has been appropriated as a versatile
tool for editing genomes, controlling gene expression, and visualizing genetic loci. To …

Kinesin-1 is a dimeric motor protein, central to intracellular transport, that steps hand-over-hand
toward the microtubule (MT) plus-end, hydrolyzing one ATP molecule per step. Its …

The response of motor proteins to external loads underlies their ability to work in teams and
determines the net speed and directionality of cargo transport. The mammalian kinesin-2, …

Internet-based scientific communities promise a means to apply distributed, diverse human
intelligence toward previously intractable scientific problems. However, current …

RNA-binding proteins (RBPs) control the fate of nearly every transcript in a cell. However,
no existing approach for studying these posttranscriptional gene regulators combines …

High-throughput methodologies have enabled routine generation of RNA target sets and
sequence motifs for RNA-binding proteins (RBPs). Nevertheless, quantitative approaches are …

Riboswitches that couple binding of ligands to conformational changes offer sensors and
control elements for RNA synthetic biology and medical biotechnology. However, design of …

Homodimeric KIF17 and heterotrimeric KIF3AB are processive, kinesin-2 family motors that
act jointly to carry out anterograde intraflagellar transport (IFT), ferrying cargo along …