Oncogene amplification on extrachromosomal DNA (ecDNA) is a common event, driving
aggressive tumor growth, drug resistance and shorter survival. Currently, the impact of …

Extrachromosomal DNA (ecDNA) is prevalent in human cancers and mediates high expression
of oncogenes through gene amplification and altered gene regulation 1 . Gene induction …

Motivation: High-throughput screens (HTS) by RNAi or small molecules are among the most
promising tools in functional genomics. They enable researchers to observe detailed …

We developed a chemically inducible Cas9 (ciCas9) and a droplet digital PCR assay for
double-strand breaks (DSB-ddPCR) to investigate the kinetics of Cas9-mediated generation …

We studied mechanisms of resistance to the novel taxane cabazitaxel in established cellular
models of taxane resistance. We also developed cabazitaxel-resistant variants from MCF-7 …

CRISPR-Cas9 nucleases are powerful genome engineering tools, but unwanted cleavage
at off-target and previously edited sites remains a major concern. Numerous strategies to …

Until recently, patients with aortic insufficiency and congestive heart failure have been offered
only the temporary palliation provided by rest, diet, cardiac glycosides, and diuretics. A …

In recent years the number of high-speed craft propelled by surface piercing propellers has
increased substantially. There are now several thousand vessels operating with surface …

Data obtained from a patient with polycythemia secondary to a patent ductus arteriosus with
reverse flow, have been presented. Oxygen saturation and tension of brachial arterial blood …

In dog experiments, angiotensin II and norepinephrine were compared in regard to some
components of the generalized pressor response each of these agents produces. It was found …