User profiles for John T. Heap
 | John HeapSchool of Life Sciences, University of Nottingham Verified email at nottingham.ac.uk Cited by 5438 |
The role of toxin A and toxin B in Clostridium difficile infection
Clostridium difficile infection is the leading cause of healthcare-associated diarrhoea in
Europe and North America 1 , 2 . During infection, C. difficile produces two key virulence …
Europe and North America 1 , 2 . During infection, C. difficile produces two key virulence …
The ClosTron: a universal gene knock-out system for the genus Clostridium
Progress in exploiting clostridial genome information has been severely impeded by a general
lack of effective methods for the directed inactivation of specific genes. Those few mutants …
lack of effective methods for the directed inactivation of specific genes. Those few mutants …
A modular system for Clostridium shuttle plasmids
JT Heap, OJ Pennington, ST Cartman… - Journal of microbiological …, 2009 - Elsevier
Despite their medical and industrial importance, our basic understanding of the biology of the
genus Clostridium is rudimentary in comparison to their aerobic counterparts in the genus …
genus Clostridium is rudimentary in comparison to their aerobic counterparts in the genus …
The ClosTron: mutagenesis in Clostridium refined and streamlined
The recent development of the ClosTron Group II intron directed mutagenesis tool for
Clostridium has advanced genetics in this genus, and here we present several significant …
Clostridium has advanced genetics in this genus, and here we present several significant …
Precise Manipulation of the Clostridium difficile Chromosome Reveals a Lack of Association between the tcdC Genotype and Toxin Production
ST Cartman, ML Kelly, D Heeg, JT Heap… - Applied and …, 2012 - Am Soc Microbiol
Clostridium difficile causes a potentially fatal diarrheal disease through the production of its
principal virulence factors, toxin A and toxin B. The tcdC gene is thought to encode a …
principal virulence factors, toxin A and toxin B. The tcdC gene is thought to encode a …
[HTML][HTML] Review of NAD (P) H-dependent oxidoreductases: Properties, engineering and application
NAD(P)H-dependent oxidoreductases catalyze the reduction or oxidation of a substrate
coupled to the oxidation or reduction, respectively, of a nicotinamide adenine dinucleotide …
coupled to the oxidation or reduction, respectively, of a nicotinamide adenine dinucleotide …
Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker
Most bacteria can only be transformed with circular plasmids, so robust DNA integration
methods for these rely upon selection of single-crossover clones followed by counter-selection …
methods for these rely upon selection of single-crossover clones followed by counter-selection …
Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum
E Steiner, AE Dago, DI Young, JT Heap… - Molecular …, 2011 - Wiley Online Library
The phosphorylated Spo0A transcription factor controls the initiation of endospore formation
in Clostridium acetobutylicum, but genes encoding key phosphorelay components, Spo0F …
in Clostridium acetobutylicum, but genes encoding key phosphorelay components, Spo0F …
The emergence of 'hypervirulence'in Clostridium difficile
The impact of Clostridium difficile-associated disease (CDAD) in healthcare settings
throughout the developed world is considerable in terms of mortality, morbidity, and disease …
throughout the developed world is considerable in terms of mortality, morbidity, and disease …
SleC Is Essential for Germination of Clostridium difficile Spores in Nutrient-Rich Medium Supplemented with the Bile Salt Taurocholate
Clostridium difficile is the major cause of infectious diarrhea and a major burden to health
care services. The ability of this organism to form endospores plays a pivotal role in infection …
care services. The ability of this organism to form endospores plays a pivotal role in infection …