The scope of targets investigated in pharmaceutical research is continuously moving into
uncharted territory. Consequently, finding suitable chemical matter with current compound …

Cellular therapies remain constrained by the limited availability of sensors for disease markers.
Here we present an integrated target-to-receptor pipeline for constructing a customizable …

Adenylyl cyclase 9 (AC9) is a membrane-bound enzyme that converts ATP into cAMP. The
enzyme is weakly activated by forskolin, fully activated by the G protein Gαs subunit and is …

How morphogen gradients control patterning and growth in developing tissues remains largely
unknown due to lack of tools manipulating morphogen gradients. Here, we generate two …

Compensatory mechanisms, such as relief of AKT-ErbB3-negative feedback, are known to
desensitize ErbB2-dependent tumours to targeted therapy. Here we describe an adaptation …

The deacetylase HDAC6 has tandem catalytic domains and a zinc finger domain (ZnF)
binding ubiquitin (Ub). While the catalytic domain has an antiviral effect, the ZnF facilitates …

The actin cytoskeleton is of fundamental importance for cellular structure and plasticity.
However, abundance and function of filamentous actin in the nucleus are still controversial. Here …

The V3 loop of the HIV-1 envelope (Env) protein elicits a vigorous, but largely non-neutralizing
antibody response directed to the V3-crown, whereas rare broadly neutralizing antibodies …

Aggregation is an important concern for therapeutic antibodies, since it can lead to reduced
bioactivity and increase the risk of immunogenicity. In our analysis of immunoglobulin G (IgG…

In this study, we characterize Designed Ankyrin Repeat Proteins (DARPins) as investigative
tools to probe botulinum neurotoxin A1 (BoNT/A1) structure and function. We identify …