Chromosome mis-segregation during mitosis leads to aneuploidy, which is a hallmark of
cancer and linked to cancer genome evolution. Errors can manifest as "lagging chromosomes" …

To infer the parameters of mechanistic models with intractable likelihoods, techniques such
as approximate Bayesian computation (ABC) are increasingly being adopted. One of the …

Current models infer that the microtubule-based mitotic spindle is built from GDP-tubulin
with small GTP caps at microtubule plus-ends, including those that attach to kinetochores, …

Stochastic simulation methods can be applied successfully to model exact spatio-temporally
resolved reaction–diffusion systems. However, in many cases, these methods can quickly …

Imaging data has become an essential tool to explore key biological questions at various
scales, for example the motile behaviour of bacteria or the transport of mRNA, and it has the …

Motivation Lattice light-sheet microscopy (LLSM) is revolutionizing cell biology since it
enables fast, high-resolution extended imaging in three dimensions combined with a drastic …

Chromosome mis-segregation during mitosis leads to daughter cells with deviant
karyotypes (aneuploidy) and an increased mutational burden through chromothripsis of mis-…

Robust control of gene expression in both space and time is of central importance in the
regulation of cellular processes and for multicellular development. However, the mechanisms by …

Mitotic chromosome segregation is a self-organising process that achieves high fidelity
separation of 46 duplicated chromosomes into two daughter cells. Chromosomes must be …

This protocol measures the 3D Euclidean distance (Δ 3D ) between two/three fluorescently
labeled kinetochore components in fixed samples using Kinetochore Delta software (KiDv1.…