Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating,
perturbing, and engineering cellular systems. Here, we develop a method for controlling gene …

While the catalog of mammalian transcripts and their expression levels in different cell types
and disease states is rapidly expanding, our understanding of transcript function lags behind…

Communication between organelles is an important feature of all eukaryotic cells. To uncover
components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened …

The availability of complete genomic sequences and technologies that allow comprehensive
analysis of global expression profiles of messenger RNA 1 , 2 , 3 have greatly expanded …

Techniques for systematically monitoring protein translation have lagged far behind
methods for measuring messenger RNA (mRNA) levels. Here, we present a ribosome-profiling …

MicroRNAs (miRNAs) are endogenous ∼22-nucleotide RNAs that mediate important gene-regulatory
events by pairing to the mRNAs of protein-coding genes to direct their repression. …

The unfolded protein response (UPR) allows the endoplasmic reticulum (ER) to recover
from the accumulation of misfolded proteins, in part by increasing its folding capacity. Inositol-…

Recent studies of transcription have revealed a level of complexity not previously appreciated
even a few years ago, both in the intricate use of post-initiation control and the mass …

Despite the essential roles of sphingolipids both as structural components of membranes
and critical signalling molecules, we have a limited understanding of how cells sense and …

A fundamental goal of cell biology is to define the functions of proteins in the context of
compartments that organize them in the cellular environment. Here we describe the construction …