Tripartite motif (TRIM) proteins make up a large family of coiled-coil-containing RING E3 ligases
that function in many cellular processes, particularly innate antiviral response pathways. …

Efficient precision genome engineering requires high frequency and specificity of integration
at the genomic target site. Here, we describe a set of resources to streamline reporter gene …

CRISPR and CRISPR-Cas effector proteins enable the targeting of DNA double-strand breaks
to defined loci based on a variable length RNA guide specific to each effector. The guide …

Transcriptional adaptation is a recently described phenomenon by which a mutation in one
gene leads to the transcriptional modulation of related genes, termed adapting genes. At the …

The ability to regulate gene activity spatially and temporally is essential to investigate cell-type-specific
gene function during development and in postembryonic processes and disease …

Transgenerational epigenetic inheritance (TEI) is mostly discussed in the context of
physiological or environmental factors. Here, we show intergenerational and transgenerational …

Efficient precision genome engineering requires high frequency and specificity of integration
at the genomic target site. Multiple design strategies for zebrafish gene targeting have …

We previously reported efficient precision targeted integration of reporter DNA in zebrafish
and human cells using CRISPR/Cas9 and short regions of homology. Here, we apply this …

Genetic robustness can be achieved via several mechanisms including transcriptional
adaptation (TA), a sequence similarity-driven process whereby mutant mRNA degradation …

The discovery and development of DNA-editing nucleases (Zinc Finger Nucleases, TALENs,
CRISPR/Cas systems) has given scientists the ability to precisely engineer or edit genomes …