Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

…, DC Baulcombe, G Peltier, JL Crespo… - ACS synthetic …, 2018 - ACS Publications
Microalgae are regarded as promising organisms to develop innovative concepts based on
their photosynthetic capacity that offers more sustainable production than heterotrophic hosts…

[PDF][PDF] Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control

…, E Jacinto, S Wullschleger, A Lorberg, JL Crespo… - Molecular cell, 2002 - cell.com
The target of rapamycin (TOR) proteins in Saccharomyces cerevisiae, TOR1 and TOR2,
redundantly regulate growth in a rapamycin-sensitive manner. TOR2 additionally regulates …

The role of TOR in autophagy regulation from yeast to plants and mammals

…, ME Pérez-Pérez, FJ Florencio, JL Crespo - Autophagy, 2008 - Taylor & Francis
The target of rapamycin (TOR) is a conserved Ser/Thr kinase that controls cell growth by
activating an array of anabolic processes including protein synthesis, transcription, and …

Evidence for a Role of VIPP1 in the Structural Organization of the Photosynthetic Apparatus in Chlamydomonas

…, A Krieger-Liszkay, H Lokstein, JL Crespo… - The Plant …, 2012 - academic.oup.com
The vesicle-inducing protein in plastids (VIPP1) was suggested to play a role in thylakoid
membrane formation via membrane vesicles. As this functional assignment is under debate, …

Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas

…, E Martínez-Force, ME Pérez-Pérez, JL Crespo - Plant …, 2018 - academic.oup.com
Fatty acids are synthesized in the stroma of plant and algal chloroplasts by the fatty acid
synthase complex. Newly synthesized fatty acids are then used to generate plastidial lipids that …

Inhibition of Target of Rapamycin Signaling and Stress Activate Autophagy in Chlamydomonas reinhardtii

ME Pérez-Pérez, FJ Florencio, JL Crespo - Plant physiology, 2010 - academic.oup.com
Autophagy is a catabolic membrane-trafficking process whereby cells recycle cytosolic proteins
and organelles under stress conditions or during development. This degradative process …

Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtii

ME Pérez-Pérez, I Couso, JL Crespo - Autophagy, 2012 - Taylor & Francis
All aerobic organisms have developed sophisticated mechanisms to prevent, detect and
respond to cell damage caused by the unavoidable production of reactive oxygen species (ROS…

Elucidating TOR Signaling and Rapamycin Action: Lessons from Saccharomyces cerevisiae

JL Crespo, MN Hall - Microbiology and Molecular Biology Reviews, 2002 - Am Soc Microbiol
TOR (target of rapamycin) is a phosphatidylinositol kinase-related protein kinase that controls
cell growth in response to nutrients. Rapamycin is an immunosuppressive and anticancer …

The TOR-controlled transcription activators GLN3, RTG1, and RTG3 are regulated in response to intracellular levels of glutamine

JL Crespo, T Powers, B Fowler… - Proceedings of the …, 2002 - National Acad Sciences
The essential, rapamycin-sensitive TOR kinases regulate a diverse set of cell growth-related
readouts in response to nutrients. Thus, the yeast TOR proteins function as nutrient sensors, …

Conditional Depletion of the Chlamydomonas Chloroplast ClpP Protease Activates Nuclear Genes Involved in Autophagy and Plastid Protein Quality Control

…, M Pellegrini, ME Perez-Perez, JL Crespo… - The Plant …, 2014 - academic.oup.com
Plastid protein homeostasis is critical during chloroplast biogenesis and responses to changes
in environmental conditions. Proteases and molecular chaperones involved in plastid …