Up-regulation of heat shock proteins is essential for cold survival during insect diapause

JP Rinehart, A Li, GD Yocum… - Proceedings of the …, 2007 - National Acad Sciences
Diapause, the dormancy common to overwintering insects, evokes a unique pattern of gene
expression. In the flesh fly, most, but not all, of the fly's heat shock proteins (Hsps) are up-…

Continuous up-regulation of heat shock proteins in larvae, but not adults, of a polar insect

JP Rinehart, SAL Hayward… - Proceedings of the …, 2006 - National Acad Sciences
Antarctica's terrestrial environment is a challenge to which very few animals have adapted.
The largest, free-living animal to inhabit the continent year-round is a flightless midge, …

[HTML][HTML] Genome of the Asian longhorned beetle (Anoplophora glabripennis), a globally significant invasive species, reveals key functional and evolutionary …

…, MF Poelchau, H Quan, J Qu, AM Ray, JP Rinehart… - Genome biology, 2016 - Springer
Background Relatively little is known about the genomic basis and evolution of wood-feeding
in beetles. We undertook genome sequencing and annotation, gene expression assays, …

[HTML][HTML] A model species for agricultural pest genomics: the genome of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

…, M Petek, MF Poelchau, É Record, JP Rinehart… - Scientific reports, 2018 - nature.com
The Colorado potato beetle is one of the most challenging agricultural pests to manage. It
has shown a spectacular ability to adapt to a variety of solanaceaeous plants and variable …

Thermotolerance and rapid cold hardening ameliorate the negative effects of brief exposures to high or low temperatures on fecundity in the flesh fly, Sarcophaga …

JP Rinehart, GD Yocum… - Physiological …, 2000 - Wiley Online Library
Although the immediate effects of temperature stress are well documented, the longer‐term
effects of such stresses are more poorly known. In these experiments, we investigate the …

Developmental upregulation of inducible hsp70 transcripts, but not the cognate form, during pupal diapause in the flesh fly, Sarcophaga crassipalpis

JP Rinehart, GD Yocum, DL Denlinger - Insect biochemistry and molecular …, 2000 - Elsevier
Partial clones of the Sarcophaga crassipalpis heat shock protein 70 (hsp70) and of heat
shock cognate 70 (hsc70) were developed by RT-PCR and library screening respectively. …

Heat‐shock protein 90 is down‐regulated during pupal diapause in the flesh fly, Sarcophaga crassipalpis, but remains responsive to thermal stress

JP Rinehart, DL Denlinger - Insect molecular biology, 2000 - Wiley Online Library
Heat‐shock protein 23 (hsp23) and hsp70 are both known to be strongly up‐regulated during
pupal diapause in the flesh fly Sarcophaga crassipalpis. This prompted us to investigate …

Diapause-specific gene expression in the northern house mosquito, Culex pipiens L., identified by suppressive subtractive hybridization

RM Robich, JP Rinehart, LJ Kitchen… - Journal of Insect …, 2007 - Elsevier
In this study we probe the molecular events underpinning diapause observed in overwintering
females of Culex pipiens. Using suppressive subtractive hybridization (SSH) we have …

Upregulation of two actin genes and redistribution of actin during diapause and cold stress in the northern house mosquito, Culex pipiens

M Kim, RM Robich, JP Rinehart… - Journal of insect …, 2006 - Elsevier
Two actin genes cloned from Culex pipiens L. are upregulated during adult diapause.
Though actins 1 and 2 were expressed throughout diapause, both genes were most highly …

Rapid cold-hardening increases the freezing tolerance of the Antarctic midge Belgica antarctica

RE Lee Jr, MA Elnitsky, JP Rinehart… - Journal of …, 2006 - journals.biologists.com
Rapid cold-hardening (RCH) is well known to increase the tolerance of chilling or cold shock
in a diverse array of invertebrate systems at both organismal and cellular levels. Here, we …