Mammalian tissues are fuelled by circulating nutrients, including glucose, amino acids, and
various intermediary metabolites. Under aerobic conditions, glucose is generally assumed to …

Autophagy is a process of self-cannibalization. Cells capture their own cytoplasm and
organelles and consume them in lysosomes. The resulting breakdown products are inputs to …

ATP is the dominant energy source in animals for mechanical and electrical work (for example,
muscle contraction or neuronal firing). For chemical work, there is an equally important …

Mutations in the enzyme cytosolic isocitrate dehydrogenase 1 (IDH1) are a common feature
of a major subset of primary human brain cancers. These mutations occur at a single amino …

Viruses rely on the metabolic network of their cellular hosts to provide energy and building
blocks for viral replication. We developed a flux measurement approach based on liquid …

The somatic mutations in cytosolic isocitrate dehydrogenase 1 (IDH1) observed in gliomas
can lead to the production of 2-hydroxyglutarate (2HG). Here, we report that tumor 2HG is …

Absolute metabolite concentrations are critical to a quantitative understanding of cellular
metabolism, as concentrations impact both the free energies and rates of metabolic reactions. …

Macropinocytosis is a highly conserved endocytic process by which extracellular fluid and
its contents are internalized into cells through large, heterogeneous vesicles known as …

One-carbon (1C) metabolism, mediated by the folate cofactor, supports multiple physiological
processes. These include biosynthesis (purines and thymidine), amino acid homeostasis (…

Autophagy is a catabolic pathway used by cells to support metabolism in response to starvation
and to clear damaged proteins and organelles in response to stress. We report here that …