The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects.
Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher …

Although several high-fidelity SpCas9 variants have been reported, it has been observed
that this increased specificity is associated with reduced on-target activity, limiting the …

Prime editors (PEs) are powerful tools that widen the possibilities for sequence modifications
during genome editing. Although methods based on the analysis of Cas9 nuclease or …

The human pathogen Pseudomonas aeruginosa uses N-butyryl-L-homoserine lactone (BHL)
and N-(3-oxododecanyl)-L-homoserine lactone (OdDHL) as small molecule intercellular …

Cas9 has made a wide range of genomic manipulation possible. However, its specificity
continues to be a challenge. Non-canonical gRNAs and new engineered variants of Cas9 have …

We present a novel genome-wide off-target prediction method named Extru-seq and
compare it with cell-based (GUIDE-seq), in vitro (Digenome-seq), and in silico methods using …

The development of clustered regularly interspaced short palindromic repeats (CRISPR)-associated
protein 9 (Cas9) into therapeutic modalities requires the avoidance of its potentially …

Many enzymes use metal ions within their active sites to achieve enormous rate acceleration.
Understanding how metal ions mediate catalysis requires elucidation of metal ion …

Ginsenoside Re is a protopanaxatriol-type saponin extracted from the berry, leaf, stem, flower
bud, and root of Panax ginseng. In recent years, ginsenoside Re (Re) has been attracting …

Purpose: Though computational epitope prediction methods have been widely used, there
have been only limited studies conducted in the context of allergies. Our research aims to …