Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has evolved variants with
substitutions in the spike receptor-binding domain (RBD) that affect its affinity for angiotensin-…

Determining the pathogenicity of genetic variants is a critical challenge, and functional
assessment is often the only option. Experimentally characterizing millions of possible missense …

Lentiviruses can infect non-dividing cells, and various cellular transport proteins provide
crucial functions for lentiviral nuclear entry and integration. We previously showed that the viral …

Lentiviruses likely infect nondividing cells by commandeering host nuclear transport factors
to facilitate the passage of their preintegration complexes (PICs) through nuclear pore …

Nipah virus (NiV) is a deadly emerging paramyxovirus. The NiV attachment (NiV-G) and fusion
(NiV-F) envelope glycoproteins mediate both syncytium formation and viral entry. Specific …

Recent genome-wide screens have highlighted an important role for transportin 3 in human
immunodeficiency virus type 1 (HIV-1) infection and preintegration complex (PIC) nuclear …

Several groups recently coupled CRISPR perturbations and single-cell RNA-seq for pooled
genetic screens. We demonstrate that vector designs of these studies are susceptible to ∼50…

Retroviruses integrate their reverse transcribed genomes into host cell chromosomes as an
obligate step in virus replication. The nuclear envelope separates the chromosomes from the …

Retroviruses integrate into cellular DNA nonrandomly. Lentiviruses such as human
immunodeficiency virus type 1 (HIV-1) favor the bodies of active genes and gene-enriched …

The myxovirus resistance (Mx) proteins are interferon-induced dynamin GTPases that can
inhibit a variety of viruses. Recently, MxB, but not MxA, was shown to restrict HIV-1 by an …