The CRISPR (clustered regularly interspaced short palindromic repeat)–Cas9 (CRISPR-associated
nuclease 9) system is poised to transform developmental biology by providing a …

… , crossover repair occurs with sufficient frequency that we now incorporate a visible marker
in the backbone of all our donor plasmids for selecting against backbone incorporation (KM O’…

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila
genome and report that Cas9-mediated genomic modifications are efficiently transmitted …

… members of the O’Connor-Giles laboratory for … O’Connor-Giles for guidance on
software development and appreciate comments from Peter Soba and members of the O’Connor-Giles …

Gene drive systems promote the spread of genetic elements through populations by assuring
they are inherited more often than Mendelian segregation would predict (see the figure). …

… We thank the members of the Harrison, Wildonger, and O'Connor-Giles labs for invaluable
input throughout this work. Plasmids and transgenic fly lines described here are available …

Regulation of synaptic growth is fundamental to the formation and plasticity of neural circuits.
Here, we demonstrate that Nervous wreck (Nwk), a negative regulator of synaptic growth at …

Cellular diversity is a fundamental characteristic of complex organisms, and the Drosophila
CNS has proved an informative paradigm for understanding the mechanisms that create …

… Harrison MM, Wildonger J, and O’Connor-Giles KM, and grants from the National Institutes
of Health to Wildonger J (R00 NS072252) and O’Connor-Giles KM (R00 NS060985 and R01 …

Neurons communicate through Ca 2+ -dependent neurotransmitter release at presynaptic
active zones (AZs). Neurotransmitter release properties play a key role in defining information …