… To distinguish between these possibilities, Eggan et al. produced mice composed exclusively
from ES cells by tetraploid complementation and compared with them NT embryos (55). …

… We are grateful to Justin Ichida for critical reading of this manuscript, Gabriella Boulting and
Alexander Meissner for helpful discussions, and all members of the Eggan Lab for continued …

Directing the differentiation of induced pluripotent stem cells into motor neurons has allowed
investigators to develop new models of amyotrophic lateral sclerosis (ALS). However, …

The generation of pluripotent stem cells from an individual patient would enable the large-scale
production of the cell types affected by that patient's disease. These cells could in turn be …

Defined transcription factors can induce epigenetic reprogramming of adult mammalian
cells into induced pluripotent stem cells. Although DNA factors are integrated during some …

The combined activity of three transcription factors can reprogram adult cells into induced
pluripotent stem cells (iPSCs). However, the transgenic methods used for delivering …

… Dikovskaia for technical support; K. Hochedlinger and T. Holm for assistance with intracytoplasmic
oocyte injection; … KE is a Junior Fellow in the Harvard Society of Fellows. … Kevin Eggan …

We have explored the use of embryonic stem cells as an alternative to oocytes for reprogramming
human somatic nuclei. Human embryonic stem (hES) cells were fused with human …

Cloning by nuclear transfer (NT) is an inefficient process in which most clones die before birth
and survivors often display growth abnormalities. In an effort to correlate gene expression …

The developmental potential of human pluripotent stem cells suggests that they can produce
disease-relevant cell types for biomedical research. However, substantial variation has …