Globular (G)-actin, the actin monomer, assembles into polarized filaments that form networks
that can provide structural support, generate force and organize the cell. Many of these …

… (K) Line scan analysis of ARPC2 fluorescence intensity at the leading edge of cells depicted
in (J). The transparent bands depict 95% confidence intervals. For all conditions, n = 400 …

… The Ras signaling pathway was of interest for our study because K-Ras-activating
oncogenic mutations causing uncontrolled cell growth are found in the vast majority (> 90%) of …

Amyotrophic lateral sclerosis (ALS) is a progressive, fatal neurodegenerative disease
characterized by motor neuron cell death. However, not all motor neurons are equally susceptible. …

… aerogenes EA289, a Kan derivative of the MDR clinical isolate Ea27,30 and K. pneumoniae
KPC2 ST258) were stored at −80 C in glycerol (15 %, v/v). Bacteria were grown in Mueller–…

… Proteinase K treatment alone removes the outer … One fraction was treated with Proteinase
K to digest all the proteins … the mitochondria prior to Proteinase K treatment, allowing the …

… The known K-Ras mutation present in this cell line has been shown to correlate with high
polyamine uptake due to its ability to affect caveolin-mediated endocytosis, (25) which leads to …

Recent advances in machine learning have greatly enhanced automatic methods to extract
information from fluorescence microscopy data. However, current machine-learning-based …

… K), with PFN1 KO mitochondria exhibiting increased velocities and run lengths (Fig. 2K).
Similar results were obtained in experiments measuring the mobility of lysosomes (Fig. …

Here, we detail a protocol using electroporation to precisely deliver defined amounts of
purified protein into CAD cells. This method allows one million cells to be electroporated with …