INTRODUCTION Most newly synthesized proteins associate into macromolecular complexes
to become functional. Complex formation requires that subunits find each other in the …

Translation regulation occurs largely during the initiation phase. Here, we develop selective
40S footprinting to visualize initiating 40S ribosomes on endogenous mRNAs in vivo. This …

Translation efficiency varies considerably between different mRNAs, thereby impacting
protein expression. Translation of the stress response master-regulator ATF4 increases upon …

Severe forms of dilated cardiomyopathy (DCM) are associated with point mutations in the
alternative splicing regulator RBM20 that are frequently located in the arginine/serine-rich …

N-terminal (Nt) acetylation is a highly prevalent co-translational protein modification in
eukaryotes, catalyzed by at least five Nt acetyltransferases (Nats) with differing specificities. Nt …

The formation of multi-protein complexes is a key feature of the cellular proteome in all kingdoms
of life. The biogenesis of protein complexes in vivo is still poorly understood, but recent …

Protein complexes are pivotal to most cellular processes. Emerging evidence indicates that
pairs of ribosomes ubiquitously drive the synchronized synthesis and assembly of two …

Translation regulation occurs largely during initiation. Currently, translation initiation can be
studied in vitro, but these systems lack features present in vivo and on endogenous mRNAs. …

Microscopy and fluorescence‐activated cell sorting (FACS) are two of the most important
tools for single‐cell phenotyping in basic and biomedical research. Microscopy provides high‐…

… We acknowledge Karina Barenhoff and Alexa Weinmann for technical assistance, Kai
Fenzl for performing qPCR experiments, Guanlin Li for a review of code, and two anonymous …