Characterizing the movement, interactions, and chemical microenvironment of a protein inside
the living cell is crucial to a detailed understanding of its function. Most strategies aimed …

Live-cell fluorescence nanoscopy is a powerful tool to study cellular biology on a molecular
scale, yet its use is held back by the paucity of suitable fluorescent probes. Fluorescent …

We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent
brightness and photostability for fluorescence imaging of actin and tubulin in living cells. …

Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

The ability to specifically attach chemical probes to individual proteins represents a powerful
approach to the study and manipulation of protein function in living cells. It provides a simple…

The visualization of complex cellular processes involving multiple proteins requires the use
of spectroscopically distinguishable fluorescent reporters. We have previously introduced the …

The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can
be implemented flexibly in living cells and in vivo. However, the design of synthetic …

PATTERNING matter on the nanometre scale is an important objective of current materials
chemistry and physics. It is driven by both the need to further miniaturize electronic …

We review new and established methods for the chemical modification of proteins in living
cells and highlight recent applications. The review focuses on tag-mediated protein labeling …

A general approach for the sequential labeling of fusion proteins of O 6 -alkylguanine-DNA
alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT …