The discovery and design of biologically important RNA molecules is outpacing three-dimensional
structural characterization. Here, we demonstrate that cryo-electron microscopy can …

Single-particle cryogenic electron microscopy (cryo-EM) has become a standard technique
for determining protein structures at atomic resolution 1 , 2 – 3 . However, cryo-EM studies of …

Increasingly, cryo-electron microscopy (cryo-EM) is used to determine the structures of RNA–protein
assemblies, but nearly all maps determined with this method have biologically …

RNA-protein complexes underlie numerous cellular processes including translation, splicing,
and posttranscriptional regulation of gene expression. The structures of these complexes …

Over the past decade, the Rosetta biomolecular modeling suite has informed diverse
biological questions and engineering challenges ranging from interpretation of low-resolution …

The Rosetta software for macromolecular modeling, docking and design is extensively used
in laboratories worldwide. During two decades of development by a community of …

RNA-Puzzles is a collective experiment in blind 3D RNA structure prediction. We report
here a third round of RNA-Puzzles. Five puzzles, 4, 8, 12, 13, 14, all structures of riboswitch …

The molecular mechanisms of exon definition and back-splicing are fundamental unanswered
questions in pre-mRNA splicing. Here we report cryo-electron microscopy structures of …

Elucidating the detailed process of ligand binding to a receptor is pharmaceutically
important for identifying druggable binding sites. With the ability to provide atomistic detail, …

Specimens below 50 kDa have generally been considered too small to be analyzed by
single-particle cryo-electron microscopy (cryo-EM). The high flexibility of pure RNAs makes it …