User profiles for Karen S. Sarkisyan
Karen SarkisyanSynthetic biology Verified email at imperial.ac.uk Cited by 2412 |
[HTML][HTML] Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics
…, C Schüler, TW Chen, KS Sarkisyan… - Frontiers in molecular …, 2013 - frontiersin.org
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience.
Here we describe red, single-wavelength GECIs, “RCaMPs,” engineered from circular …
Here we describe red, single-wavelength GECIs, “RCaMPs,” engineered from circular …
Local fitness landscape of the green fluorescent protein
Fitness landscapes 1 , 2 depict how genotypes manifest at the phenotypic level and form the
basis of our understanding of many areas of biology 2 , 3 , 4 , 5 , 6 , 7 , yet their properties …
basis of our understanding of many areas of biology 2 , 3 , 4 , 5 , 6 , 7 , yet their properties …
Genetically encodable bioluminescent system from fungi
AA Kotlobay, KS Sarkisyan… - Proceedings of the …, 2018 - National Acad Sciences
Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually
oriented functions from attracting mates to scaring off predators. Half a dozen different …
oriented functions from attracting mates to scaring off predators. Half a dozen different …
[HTML][HTML] A brief review of bioluminescent systems (2019)
A Fleiss, KS Sarkisyan - Current genetics, 2019 - Springer
Despite being widely used in reporter technologies, bioluminescent systems are largely
understudied. Of at least forty different bioluminescent systems thought to exist in nature, …
understudied. Of at least forty different bioluminescent systems thought to exist in nature, …
Plants with genetically encoded autoluminescence
…, FA Kondrashov, IV Yampolsky, KS Sarkisyan - Nature …, 2020 - nature.com
Autoluminescent plants engineered to express a bacterial bioluminescence gene cluster in
plastids have not been widely adopted because of low light output. We engineered tobacco …
plastids have not been widely adopted because of low light output. We engineered tobacco …
[HTML][HTML] An experimental assay of the interactions of amino acids from orthologous sequences shaping a complex fitness landscape
…, EV Putintseva, L Espinar, KS Sarkisyan… - PLoS …, 2019 - journals.plos.org
Characterizing the fitness landscape, a representation of fitness for a large set of genotypes,
is key to understanding how genetic information is interpreted to create functional organisms…
is key to understanding how genetic information is interpreted to create functional organisms…
[HTML][HTML] Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal
…, MS Baranov, NV Klementieva, KS Sarkisyan… - Chemical …, 2017 - pubs.rsc.org
We present protein-PAINT – the implementation of the general principles of PAINT (Point
Accumulation for Imaging in Nanoscale Topography) for live-cell protein labeling. Our method …
Accumulation for Imaging in Nanoscale Topography) for live-cell protein labeling. Our method …
[HTML][HTML] KillerOrange, a genetically encoded photosensitizer activated by blue and green light
Genetically encoded photosensitizers, proteins that produce reactive oxygen species when
illuminated with visible light, are increasingly used as optogenetic tools. Their applications …
illuminated with visible light, are increasingly used as optogenetic tools. Their applications …
[PDF][PDF] Green fluorescent protein with anionic tryptophan-based chromophore and long fluorescence lifetime
KS Sarkisyan, AS Goryashchenko, PV Lidsky… - Biophysical journal, 2015 - cell.com
Spectral diversity of fluorescent proteins, crucial for multiparameter imaging, is based mainly
on chemical diversity of their chromophores. Recently we have reported, to our knowledge, …
on chemical diversity of their chromophores. Recently we have reported, to our knowledge, …
Heterogeneity of the GFP fitness landscape and data-driven protein design
Studies of protein fitness landscapes reveal biophysical constraints guiding protein evolution
and empower prediction of functional proteins. However, generalisation of these findings is …
and empower prediction of functional proteins. However, generalisation of these findings is …