Eukaryotic cells have a layer of heterochromatin at the nuclear periphery. To investigate
mechanisms regulating chromatin distribution, we analyzed heterochromatin organization in …

Specific control of gene activity is a valuable tool to study and engineer cellular functions.
Recent studies uncovered the potential of transcription activator-like effector (TALE) proteins …

Genome-wide molecular studies have provided new insights into the organization of nuclear
chromatin by revealing the presence of chromatin domains of differing transcriptional activity…

Despite the well-established role of nuclear organization in the regulation of gene expression,
little is known about the reverse: how transcription shapes the spatial organization of the …

Epigenetic regulation of gene expression involves, besides DNA and histone modifications,
the relative positioning of DNA sequences within the nucleus. To trace specific DNA …

Any profound comprehension of gene function requires detailed information about the
subcellular localization, molecular interactions and spatio-temporal dynamics of gene products. …

The spatial arrangement of chromatin is linked to the regulation of nuclear processes. One
striking aspect of nuclear organization is the spatial segregation of heterochromatic and …

Peripheral heterochromatin in mammalian nuclei is tethered to the nuclear envelope by at
least two mechanisms here referred to as the A- and B-tethers. The A-tether includes lamins A/…

Abnormalities are indispensable for studying normal biological processes and mechanisms.
In the present work, we draw attention to the remarkable phenomenon of a perpetually and …

Charge heterogeneity analysis of monoclonal antibodies (mAbs) and complex formats, such
as bispecifics, is crucial for therapeutic applications. In this study, we developed two …