We performed label-free observation of molecular dynamics in apoptotic cells by Raman
microscopy. Dynamic changes in cytochrome c distribution at the Raman band of 750 cm -1 …

In chemical biology research, various fluorescent probes have been developed and used to
visualize target proteins or molecules in living cells and tissues, yet there are limitations to …

Raman microscopy is a promising technology for visualizing the distribution of molecules in
cells. A challenge for live-cell imaging using Raman microscopy has been long imaging …

We demonstrate dynamic imaging of molecular distribution in unstained living cells using
Raman scattering. By combining slit-scanning detection and optimizing the excitation …

Alkyne has a unique Raman band that does not overlap with Raman scattering from any
endogenous molecule in live cells. Here, we show that alkyne-tag Raman imaging (ATRI) is a …

Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe,
EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne …

Dynamic SERS imaging inside a living cell is demonstrated with the use of a gold nanoparticle,
which travels through the intracellular space to probe local molecular information over …

We demonstrate the use of saturated excitation in confocal fluorescence microscopy to
improve the spatial resolution. In the proposed technique, we modulate the excitation intensity …

Highlights • Parallel detection of Raman spectra enables Raman imaging of live cells with
high spatial and temporal resolution. • Raman imaging of unstained live cells using 532 nm …

Sphingomyelin (SM) and cholesterol (chol)-rich domains in cell membranes, called lipid rafts,
are thought to have important biological functions related to membrane signaling and …