Understanding how a subset of expressed genes dictates cellular phenotype is a considerable
challenge owing to the large numbers of molecules involved, their combinatorics and the …

Although some cell types may be defined anatomically or by physiological function, a
rigorous definition of cell state remains elusive. Here, we develop a quantitative, imaging-based …

Cardiac tissue engineering is a promising approach to provide large-scale tissues for
transplantation to regenerate the heart after ischemic injury, however, integration with the host …

Regenerating the human heart is a challenge that has engaged researchers and clinicians
around the globe for nearly a century. From the repair of the first septal defect in 1953, …

We describe a multistep method for endogenous tagging of transcriptionally silent genes in
human induced pluripotent stem cells (hiPSCs). A monomeric EGFP (mEGFP) fusion tag and …

We performed a comprehensive analysis of the transcriptional changes occurring during
human induced pluripotent stem cell (hiPSC) differentiation to cardiomyocytes. Using single cell …

Despite the intimate link between cell organization and function, the principles underlying
intracellular organization and the relation between organization, gene expression and …

Current cell transplantation techniques are hindered by small graft size, requiring high cell
doses to achieve therapeutic cardiac remuscularization. Enhancing the proliferation of …

Transplantation of stem cell-derived cardiomyocytes is a promising strategy for repairing
damaged cardiac muscle following a myocardial infarction. Our group and others have …

We describe a multi-step CRISPR/Cas9 gene editing method to create endogenously tagged
GFP-fusions of transcriptionally silent genes in human induced pluripotent stem cells (…