We report ultrasensitive Ca 2+ indicators, yellow cameleon-Nano (YC-Nano), developed by
engineering the Ca 2+ -sensing domain of a genetically encoded Ca 2+ indicator, YC2.60 or …

The use of fluorescent proteins has revolutionized our understanding of biological processes.
However, the requirement for external illumination precludes their universal application to …

Periodic somite segmentation in vertebrate embryos is controlled by the ‘segmentation clock’,
which consists of numerous cellular oscillators. Although the properties of a single …

Astrocytes generate local calcium (Ca 2+ ) signals that are thought to regulate their functions.
Visualization of these signals in the intact brain requires an imaging method with high …

In teleost fish, the Mauthner (M) cell, a large reticulospinal neuron in the brainstem, triggers
escape behavior. Spinal commissural inhibitory interneurons that are electrotonically excited …

We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an
emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins …

Gene trap and enhancer trap methods using transposon or retrovirus have been recently
described in zebrafish. However, insertional mutants using these methods have not been …

DNA interstrand crosslinks (ICLs) are highly toxic lesions that stall the replication fork to
initiate the repair process during the S phase of vertebrates. Proteins involved in Fanconi …

Developing somites express two subtypes of classic cadherin adhesion receptors, N-cadherin
and cadherin-11 (cad11). To investigate the role of these adhesion molecules in somite …

cAMP is one of the most important second messengers in biological processes. Cellular
dynamics of cAMP have been investigated using a series of fluorescent indicators; however, …