From G protein-coupled receptors to ion channels, membrane proteins represent over half
of known drug targets. Yet, structure-based drug discovery is hampered by the dearth of …

The periplasmic seventeen kilodalton protein (Skp) chaperone has been characterized
primarily for its role in outer membrane protein (OMP) biogenesis, during which the jellyfish-like …

SARS-CoV-2 Omicron variant XBB.1.5 has shown extraordinary immune escape even for
fully vaccinated individuals. There are currently no approved antibodies that neutralize this …

Computational antibody engineering efforts to date have focused on improving binding
affinities or biophysical characteristics. De novo design of antibodies binding specific epitopes …

Fibroblast growth factor 18 (FGF18) is elevated in several human cancers, such as gastrointestinal
and ovarian cancers, and stimulates the proliferation of tumor cells. This suggests that …

Crystallization chaperones are attracting increasing interest as a route to crystal growth and
structure elucidation of difficult targets such as membrane proteins. While strategies to date …

Directed evolution relies on both random and site-directed mutagenesis of individual genes
and regulatory elements to create variants with altered activity profiles for engineering …

The SARS-CoV-2 Omicron variant of concern (VoC) and its sublineages contain 31–36
mutations in spike and escape neutralization by most therapeutic antibodies. In a pseudovirus …

Antibody aggregation is frequently mediated by the complementarity determining regions
within the variable domains and can significantly decrease purification yields, shorten shelf-life …

Budding yeast, Saccharomyces cerevisiae, respond to temperature changes by either
activating or repressing the transcription of certain genes. The response of budding yeast to …