Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly
interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems, that use …

RNA-guided Cas9 nucleases derived from clustered regularly interspaced short palindromic
repeats (CRISPR)-Cas systems have dramatically transformed our ability to edit the …

Mice Against Ticks is a community-guided ecological engineering project that aims to prevent
tick-borne disease by using CRISPR-based genome editing to heritably immunize the …

Prokaryotic type II CRISPR-Cas systems can be adapted to enable targeted genome
modifications across a range of eukaryotes 1 , 2 , 3 , 4 , 5 , 6 , 7 . Here we engineer this system to …

We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated
endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans …

The Cas9 protein from the Streptococcus pyogenes CRISPR-Cas acquired immune system
has been adapted for both RNA-guided genome editing and gene regulation in a variety of …

10.7554/eLife.03401.001 Gene drives may be capable of addressing ecological problems by
altering entire populations of wild organisms, but their use has remained largely theoretical …

Laboratory evolution has generated many biomolecules with desired properties, but a
single round of mutation, gene expression, screening or selection, and replication typically …

Genome‐modification technologies enable the rational engineering and perturbation of
biological systems. Historically, these methods have been limited to gene insertions or mutations …

RNA-guided gene drives capable of spreading genomic alterations made in laboratory
organisms through wild populations could be used to address environmental and public health …