Loss of Tsc1 in vivo impairs hippocampal mGluR-LTD and increases excitatory synaptic function

HS Bateup, KT Takasaki, JL Saulnier… - Journal of …, 2011 - Soc Neuroscience
The autism spectrum disorder tuberous sclerosis complex (TSC) is caused by mutations in
the Tsc1 or Tsc2 genes, whose protein products form a heterodimeric complex that negatively …

[PDF][PDF] Supraresolution imaging in brain slices using stimulated-emission depletion two-photon laser scanning microscopy

JB Ding, KT Takasaki, BL Sabatini - Neuron, 2009 - cell.com
Two-photon laser scanning microscopy (2PLSM) has allowed unprecedented fluorescence
imaging of neuronal structure and function within neural tissue. However, the resolution of …

Optically selective two-photon uncaging of glutamate at 900 nm

JP Olson, HB Kwon, KT Takasaki… - Journal of the …, 2013 - ACS Publications
We have synthesized a 7-diethylaminocoumarin (DEAC) derivative that allows wavelength-selective
two-photon uncaging at 900 nm versus 720 nm. This new caging chromophore, …

[PDF][PDF] Live-cell superresolution imaging by pulsed STED two-photon excitation microscopy

KT Takasaki, JB Ding, BL Sabatini - Biophysical journal, 2013 - cell.com
Two-photon laser scanning microscopy (2PLSM) allows fluorescence imaging in thick
biological samples where absorption and scattering typically degrade resolution and signal …

Phase-space measurement for depth-resolved memory-effect imaging

KT Takasaki, JW Fleischer - Optics express, 2014 - opg.optica.org
Random scattering of light by a turbid layer prevents conventional imaging of objects
hidden behind it. Angular correlations in the scattered light, created by the so-called optical …

Superficial bound of the depth limit of two-photon imaging in mouse brain

K Takasaki, R Abbasi-Asl, J Waters - Eneuro, 2020 - eneuro.org
Two-photon fluorescence microscopy has been used extensively to probe the structure and
functions of cells in living biological tissue. Two-photon excitation generates fluorescence …

[HTML][HTML] Super-resolution 2-photon microscopy reveals that the morphology of each dendritic spine correlates with diffusive but not synaptic properties

K Takasaki, BL Sabatini - Frontiers in neuroanatomy, 2014 - frontiersin.org
The structure of dendritic spines suggests a specialized function in compartmentalizing
synaptic signals near active synapses. Indeed, theoretical and experimental analyses indicate …

[PDF][PDF] Laminar distribution and arbor density of two functional classes of thalamic inputs to primary visual cortex

…, ND Ouellette, EE Turschak, RS Larsen, KT Takasaki… - Cell reports, 2021 - cell.com
Motion/direction-sensitive and location-sensitive neurons are the two major functional types
in mouse visual thalamus that project to the primary visual cortex (V1). It is under debate …

Dual-plane 3-photon microscopy with remote focusing

KT Takasaki, D Tsyboulski, J Waters - Biomedical Optics Express, 2019 - opg.optica.org
3-photon excitation enables in vivo fluorescence microscopy deep in densely labeled and
highly scattering samples. To date, 3-photon excitation has been restricted to scanning a …

Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues

…, E Turschak, A Liddell, J Rohde, A Hellevik, K Takasaki… - bioRxiv, 2023 - biorxiv.org
Recent advances in tissue processing, labeling, and fluorescence microscopy are providing
unprecedented views of the structure of cells and tissues at sub-diffraction resolutions and …