Experience with host cell protein impurities in biopharmaceuticals
…, F Gunawan, T Waerner, KE Van Cott - Biotechnology …, 2018 - Wiley Online Library
In the 40‐year history of biopharmaceuticals, there have been a few cases where the final
products contained residual host cell protein (HCP) impurities at levels high enough to be of …
products contained residual host cell protein (HCP) impurities at levels high enough to be of …
Analysis of the N-glycans of recombinant human Factor IX purified from transgenic pig milk
GC Gil, WH Velander, KE Van Cott - Glycobiology, 2008 - academic.oup.com
Glycosylation of recombinant proteins is of particular importance because it can play significant
roles in the clinical properties of the glycoprotein. In this work, the N-glycan structures of …
roles in the clinical properties of the glycoprotein. In this work, the N-glycan structures of …
Efficient, thermally stable, second order nonlinear optical response in organic hybrid covalent/ionic self-assembled films
A covalent/electrostatic layer-by-layer self-assembly method was used to achieve polar
ordering of a water soluble, reactive dye in the fabrication of nonlinear optical (NLO) films. We …
ordering of a water soluble, reactive dye in the fabrication of nonlinear optical (NLO) films. We …
Haemophilic factors produced by transgenic livestock: abundance that can enable alternative therapies worldwide
KE Van Cott, PE Monahan, TC Nichols… - …, 2004 - Wiley Online Library
Haemophilia replacement factors, both plasma‐derived and recombinant, are in relatively
short supply and are high‐cost products. This has stymied the study and development of …
short supply and are high‐cost products. This has stymied the study and development of …
Biosensors employing ionic self-assembled multilayers adsorbed on long-period fiber gratings
Z Wang, JR Heflin, K Van Cott, RH Stolen… - Sensors and Actuators B …, 2009 - Elsevier
We demonstrate that ionic self-assembled multilayers (ISAMs) adsorbed on long period
gratings (LPGs) function effectively as biosensors using biotin–streptavidin as a demonstration …
gratings (LPGs) function effectively as biosensors using biotin–streptavidin as a demonstration …
Tobacco protein separation by aqueous two-phase extraction
D Balasubramaniam, C Wilkinson, K Van Cott… - … of Chromatography A, 2003 - Elsevier
Tobacco has long been considered as a host to produce large quantity of high-valued
recombinant proteins. However, dealing with large quantities of biomass is a challenge for …
recombinant proteins. However, dealing with large quantities of biomass is a challenge for …
[PDF][PDF] Layer-by-layer deposition and ordering of low-molecular-weight dye molecules for second-order nonlinear optics
KE Van Cott, M Guzy, P Neyman, C Brands… - Angewandte Chemie …, 2002 - academia.edu
A combination of electrostatic interactions and covalent bonding is used to form films with
low-molecular-weight chromophores by a layer-by-layer deposition process. Using a common, …
low-molecular-weight chromophores by a layer-by-layer deposition process. Using a common, …
Current progress in the production of recombinant human fibrinogen in the milk of transgenic animals
SP Butler, K Van Cott, A Subrumanian… - Thrombosis and …, 1997 - thieme-connect.com
1.23 Kb 5'and 3'sequences Coding sequences ffi Fibrinogen introns the milk of transgenic
mice, pigs and sheep. A general cloning strategy used in fibrinogen Eansgene design …
mice, pigs and sheep. A general cloning strategy used in fibrinogen Eansgene design …
Transgenic pigs as bioreactors: a comparison of gamma-carboxylation of glutamic acid in recombinant human protein C and factor IX by the mammary gland
KE Van Cott, SP Butler, CG Russell… - Genetic analysis …, 1999 - Elsevier
The mammary gland of transgenic livestock can be used as a bioreactor for producing
complex therapeutic proteins. However, the capacity for making a given post-translational …
complex therapeutic proteins. However, the capacity for making a given post-translational …
Purification of recombinant DNA-derived factor IX produced in transgenic pig milk and fractionation of active and inactive subpopulations
…, A Cadiz, WH Velander, C Zhang, KE Van Cott - … of Chromatography A, 2004 - Elsevier
Transgenic animal bioreactors can be engineered to make gram per liter quantities of complex
recombinant glycoproteins in milk. However, little is known about the limitations in post-…
recombinant glycoproteins in milk. However, little is known about the limitations in post-…