A double-stranded DNA cut has been observed in the mating type (MAT) locus of the yeast
Saccharomyces cerevisiae in cultures undergoing homothallic cassette switching. Cutting is …

Background: DNA replication and mitosis are triggered by activation of kinase complexes,
each made up of a cyclin and a cyclin-dependent kinase (Cdk). It had seemed possible that …

cdc28, one of several genes required for cell division in the yeast Saccharomyces cerevisiae,
has been isolated on recombinant plasmids. A recombinant plasmid pool containing the …

The mating type of haploid yeast (a or alpha) is determined by information present at the MAT
locus. Identical copies of a and alpha information are present at distal loci (HMR and HML)…

The complete sequences of the yeast a mating-type locus, MATa, and of the silent a cassette,
HMLcy, have been determined. A segment of 842 nucleotides is unique to MATa, and a …

Cohesin is important for 3D genome organization. Nevertheless, even the complete removal
of cohesin has surprisingly little impact on steady-state gene transcription and enhancer …

A putative origin of DNA replication is associated with the DNA sequences necessary for the
repression of silent mating type loci in yeast. These sequences lie about a kilobase away …

Past experience in molecular biology has shown that some of the most fertile hunting grounds
for molecular biologists are highly evolved, highly efficient systems that have an obvious …

Cohesin organizes DNA into chromatids, regulates enhancer-promoter interactions, and
confers sister chromatid cohesion. Its association with chromosomes is regulated by hook-…

How chromosome organization is related to genome function remains poorly understood.
Cohesin, loop extrusion, and CCCTC-binding factor (CTCF) have been proposed to create …