Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase.
This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg+…

ATP-dependent DNA supercoiling catalyzed by Escherichia coli DNA gyrase was inhibited
by oxolinic acid, a compound similar to but more potent than nalidixic acid and a known …

Integration host factor (IHF) is a small heterodimeric protein that specifically binds to DNA
and functions as an architectural factor in many cellular processes in prokaryotes. Here, we …

Retroviral DNA integration involves a coordinated set of DNA cutting and jolning reactions.
Linear viral DNA is cleaved at each 3’end to generate the precursor ends for integration. The …

Genome rearrangements are frequent events in many organisms. Many of these events
involve specific protein factors and specific DNA sequences. A segment of DNA that has the …

Two conserved DNA sequences serve as joining signals in the assembly of immunoglobulins
and T-cell receptors from V-, (D)-, and J-coding segments during lymphoid differentiation. …

The structure of a presumptive DNA intermediate in the integration of retroviral DNA was
studied in a cellfree reaction with exogenously added target DNA. The product made by viral …

Barrier-to-autointegration factor (BAF) is a highly conserved cellular protein that was identified
by its activity in protecting retroviral DNA against autointegration. We show that BAF has …

DNA gyrase from Escherichia coli, in the presence of ATP, can both separate catenated DNA
circles and unknot knotted DNA. Both these reactions require passage of a DNA segment …

Pre-B and pre-T cell lines from mutant mice with severe combined immune deficiency (scid
mice) were transfected with plasmids that contained recombination signal sequences of …