DNA gyrase: an enzyme that introduces superhelical turns into DNA.

M Gellert, K Mizuuchi, MH O'Dea… - Proceedings of the …, 1976 - National Acad Sciences
Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase.
This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg+…

Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity

M Gellert, K Mizuuchi, MH O'Dea… - Proceedings of the …, 1977 - National Acad Sciences
ATP-dependent DNA supercoiling catalyzed by Escherichia coli DNA gyrase was inhibited
by oxolinic acid, a compound similar to but more potent than nalidixic acid and a known …

[HTML][HTML] Crystal structure of an IHF-DNA complex: a protein-induced DNA U-turn

PA Rice, S Yang, K Mizuuchi, HA Nash - Cell, 1996 - cell.com
Integration host factor (IHF) is a small heterodimeric protein that specifically binds to DNA
and functions as an architectural factor in many cellular processes in prokaryotes. Here, we …

HIV-1 DNA integration: mechanism of viral DNA cleavage and DNA strand transfer

A Engelman, K Mizuuchi, R Craigie - Cell, 1991 - cell.com
Retroviral DNA integration involves a coordinated set of DNA cutting and jolning reactions.
Linear viral DNA is cleaved at each 3’end to generate the precursor ends for integration. The …

Transpositional recombination: mechanistic insights from studies of Mu and other elements

K Mizuuchi - Annual review of biochemistry, 1992 - annualreviews.org
Genome rearrangements are frequent events in many organisms. Many of these events
involve specific protein factors and specific DNA sequences. A segment of DNA that has the …

V (D) J recombination: a functional definition of the joining signals.

JE Hesse, MR Lieber, K Mizuuchi… - Genes & …, 1989 - genesdev.cshlp.org
Two conserved DNA sequences serve as joining signals in the assembly of immunoglobulins
and T-cell receptors from V-, (D)-, and J-coding segments during lymphoid differentiation. …

Retroviral DNA integration: structure of an integration intermediate

T Fujiwara, K Mizuuchi - Cell, 1988 - cell.com
The structure of a presumptive DNA intermediate in the integration of retroviral DNA was
studied in a cellfree reaction with exogenously added target DNA. The product made by viral …

Barrier-to-autointegration factor (BAF) bridges DNA in a discrete, higher-order nucleoprotein complex

…, R Ghirlando, MS Lee, K Mizuuchi… - Proceedings of the …, 2000 - National Acad Sciences
Barrier-to-autointegration factor (BAF) is a highly conserved cellular protein that was identified
by its activity in protecting retroviral DNA against autointegration. We show that BAF has …

DNA gyrase action involves the introduction of transient double-strand breaks into DNA.

K Mizuuchi, LM Fisher, MH O'Dea… - Proceedings of the …, 1980 - National Acad Sciences
DNA gyrase from Escherichia coli, in the presence of ATP, can both separate catenated DNA
circles and unknot knotted DNA. Both these reactions require passage of a DNA segment …

The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V (D) J recombination

…, S Lewis, GC Bosma, N Rosenberg, K Mizuuchi… - Cell, 1988 - cell.com
Pre-B and pre-T cell lines from mutant mice with severe combined immune deficiency (scid
mice) were transfected with plasmids that contained recombination signal sequences of …