CRISPR-Cas9 is widely used in genomic editing, but the kinetics of target search and its
relation to the cellular concentration of Cas9 have remained elusive. Effective target search …

METHODS Data analysis in SMLM consists of the following steps: Identifying potential
molecules and selecting regions of interests (ROIs) around their approximate localization, sub-…

CRISPR-Cas systems encode RNA-guided surveillance complexes to find and cleave invading
DNA elements. While it is thought that invaders are neutralized minutes after cell entry, …

Single-molecule localization microscopy (SMLM) is a powerful super-resolution technique
for elucidating structure and dynamics in the life- and material sciences. Simultaneously …

CRISPR-Cas systems have widely been adopted as genome editing tools, with two frequently
employed Cas nucleases being SpyCas9 and LbCas12a. Although both nucleases use …

Single-molecule localization microscopy (SMLM) is an advanced microscopy method that
uses the blinking of fluorescent molecules to determine the position of these molecules with a …

Hydrogels made of the polysaccharide κ-carrageenan are widely used in the food and
personal care industry as thickeners or gelling agents. These hydrogels feature dense regions …

The techniques that are currently available to assess fat crystal networks are compromised
with respect to invasive sample preparation and ability to quantify compositional and …

A detailed investigation was carried out on the modulation of the coupling between network
formation and the recrystallization of oil-dispersed micronized fat crystal (MFC) nanoplatelets …

In single-molecule localization microscopy (SMLM), the use of engineered point spread
functions (PSFs) provides access to three-dimensional localization information. The …