The CRISPR–Cas system is a powerful genome editing tool that functions in a diverse array
of organisms and cell types. The technology was initially developed to induce targeted …

In just 3 years CRISPR genome editing has transformed biology, and its popularity and potency
continue to grow. New CRISPR effectors and rules for locating optimum targets continue …

Polyadenylation at the 3′-end is a major regulator of messenger RNA and its length is
known to affect nuclear export, stability, and translation, among others. Only recently have …

The design of optimal guide RNA (gRNA) sequences for CRISPR systems is challenged by
the need to achieve highly efficient editing at the desired location (on‐target editing) with …

We present ampliCan, an analysis tool for genome editing that unites highly precise
quantification and visualization of genuine genome editing events. ampliCan features nuclease-…

Background With the rapid growth in the use of high-throughput methods for characterizing
translation and the continued expansion of multi-omics, there is a need for back-end …

Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR)
to introduce custom DNA sequences to target sites. The HDR editing efficiency varies …

The rate of translation can vary depending on the mRNA template. During the elongation
phase the ribosome can transiently pause or permanently stall. A pause can provide the …

RNA molecules can form secondary and tertiary structures that can regulate their
localization and function. Using enzymatic or chemical probing together with high-throughput …

Motivation: The search for causative genetic variants in rare diseases of presumed monogenic
inheritance has been boosted by the implementation of whole exome (WES) and whole …